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cas no 66 81 9 bglii restriction enzyme new england biolabs  (New England Biolabs)


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    New England Biolabs cas no 66 81 9 bglii restriction enzyme new england biolabs
    Cas No 66 81 9 Bglii Restriction Enzyme New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cas no 66 81 9 bglii restriction enzyme new england biolabs/product/New England Biolabs
    Average 97 stars, based on 2796 article reviews
    cas no 66 81 9 bglii restriction enzyme new england biolabs - by Bioz Stars, 2026-06
    97/100 stars

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    Effect of CRISPR-Cas9 mediated LINC00511 promoter deletion on HCC70 colony formation and paclitaxel sensitivity. ( a , b ) Genomic deletion of the LINC00511 promoter using CRISPR-Cas9 in the HCC70 TNBC model. qRT-PCR for LINC00511 expression in HCC70 WT and LINC00511-KO cell models. Data are presented as mean ± SD, n = 3. *** p < 0.0005. Clonogenic assay for HCC70 parental and LINC00511-KO cells, in the absence ( c , d ) or the presence ( e , f ) of indicated concentrations of Paclitaxel. Data are presented as mean ± SD, n = 4. *** p < 0.0005. ( g ) Cell cycle analysis for HCC70 parental and LINC00511-KO cells in the absence or presence of different concentrations of Paclitaxel.

    Journal: Non-Coding RNA

    Article Title: LncRNA-Based Classification of Triple Negative Breast Cancer Revealed Inherent Tumor Heterogeneity and Vulnerabilities

    doi: 10.3390/ncrna8040044

    Figure Lengend Snippet: Effect of CRISPR-Cas9 mediated LINC00511 promoter deletion on HCC70 colony formation and paclitaxel sensitivity. ( a , b ) Genomic deletion of the LINC00511 promoter using CRISPR-Cas9 in the HCC70 TNBC model. qRT-PCR for LINC00511 expression in HCC70 WT and LINC00511-KO cell models. Data are presented as mean ± SD, n = 3. *** p < 0.0005. Clonogenic assay for HCC70 parental and LINC00511-KO cells, in the absence ( c , d ) or the presence ( e , f ) of indicated concentrations of Paclitaxel. Data are presented as mean ± SD, n = 4. *** p < 0.0005. ( g ) Cell cycle analysis for HCC70 parental and LINC00511-KO cells in the absence or presence of different concentrations of Paclitaxel.

    Article Snippet: In brief, 1000 ng of each gRNA were mixed with 1.5 μL of 20 µM Cas-9 Enzyme (EnGen Spy Cas9 NLS (NEB# M0646M)) to form the RNP complex.

    Techniques: CRISPR, Quantitative RT-PCR, Expressing, Clonogenic Assay, Cell Cycle Assay